Reversion of Streptococcal Protoplasts

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Reversion to the streptococcal state of enterococcal protoplasts, spheroplasts, and L-forms.

A method is described for predetermining whether lysozyme-damaged enterococci grow as either the parent strain or as L-forms. Organisms treated with lysozyme grew as L-forms on media solidified with low concentrations of agar, or the damaged cells grew as streptococci on media solidified with high concentrations of gelatin. After induction, some of the L-forms reverted to the parent strain, but...

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Reversion of Bacillus megaterium protoplasts to the bacillary form.

Photomicrographic evidence of reversion of Bacillus megaterium protoplasts to the bacillary form on soft agar plates hypertonic medium is demonstrated.

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Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: photomicrographic study.

When lysozyme protoplasts of Bacillus subtilis 168 indoleare washed free of lysozyme and plated on hypertonic soft agar medium, each protoplast forms an L colony. The L colonies consist of cell wall-free L bodies which, in turn, give rise to new L colonies. This heritably propagated wall-less condition can be terminated promptl"'6 by plating the cells in 25%, gelatin medium. In this&t;environme...

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Morphological study of the reversion to bacillary form, of Bacillus megaterium protoplasts.

Protoplasts of Bacillus megaterium readily reverted to bacillary form in liquid media and when plated in a soft-agar layer onto the surface of appropriate agar media. Three phases of the reversion sequence could be differentiated by phase contrast microscopy: (i) increase in size of the individual protoplasts, (ii) non oriented division of the protoplasts and (iii) outgrowth of the bacillary fo...

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Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: biosynthesis of macromolecules and wall during successive steps.

Protoplasts of Bacillus subtilis plated on SDG medium formed L colonies in quantative yield and propagated in the L-form indefinitely. Protoplasts or L bodies placed in 25% gelatin medium formed bacillary colonies. Details of the reversion of these naked bodies to the walled form are reported here. Protoplasts prepared in minimal medium reverted fairly synchronously 3 to 4 hr after inoculation ...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1972

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.112.1.611-614.1972